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Human IL-12 Protein 4732

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
SKU: 4732parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P29460(IL2B)&P29459(IL2A)

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

molecular weight

The protein has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-12 Protein 4732

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

ELISA with Human IL-12 Protein
Immobilized Human IL-12, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).
Cell based assay with Human IL-12 Protein
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1 - 1 ng/mL.
HPLC of Human IL-12 Protein
The purity of Human IL-12 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-12 Protein
Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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