Skip to content

Human IL-12 Protein 4732

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
SKU: 4732parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P29460(IL2B)&P29459(IL2A)

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

molecular weight

The protein has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-12 Protein 4732

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Protein names
Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40) (NK cell stimulatory factor chain 2) (NKSF2)
Gene names
IL12B,IL12B NKSF2
Protein family
IL-12B family
Mass
9606Da
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Subellular location
Secreted.
Structure
Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer. Interacts with NBR1; this interaction promotes IL-12 secretion (By similarity).
Post-translational modification
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Target Relevance information above includes information from UniProt accession: P29460
The UniProt Consortium

Data

ELISA with Human IL-12 Protein
Immobilized Human IL-12, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).
Cell based assay with Human IL-12 Protein
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1 - 1 ng/mL.
HPLC of Human IL-12 Protein
The purity of Human IL-12 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-12 Protein
Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information.

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.