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Human ICOS/CD278 (C136S, C137S) Protein 4629

$330.00$1,100.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu21-Phe141(C136S,C137S)
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9Y6W8

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

molecular weight

The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human ICOS/CD278 (C136S, C137S) Protein 4629

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Protein names
Inducible T-cell costimulator (Activation-inducible lymphocyte immunomediatory molecule) (CD antigen CD278)
Gene names
ICOS,ICOS AILIM
Mass
9606Da
Function
FUNCTION: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity). {ECO:0000250, ECO:0000269|PubMed:11169414, ECO:0000269|PubMed:9930702}.
Subellular location
SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. {ECO:0000269|PubMed:11006126, ECO:0000269|PubMed:11169414, ECO:0000269|PubMed:9930702}.
Structure
SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:11169414, ECO:0000269|PubMed:9930702}.
Post-translational modification
PTM: N-glycosylated. {ECO:0000269|PubMed:11169414}.
Target Relevance information above includes information from UniProt accession : Q9Y6W8
The UniProt Consortium

Data

SPR with Human ICOS/CD278 (C136S, C137S) Protein
Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
ELISA with Human ICOS/CD278 (C136S, C137S) Protein
Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test).
SDS-PAGE gel of Human ICOS/CD278 (C136S, C137S) Protein
Human ICOS (C136S,C137S) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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