| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| accession | AAV53343(HLA-A*11:01)&P61769(B2M)&VVVGAGGVGK |
| express system | HEK293 |
| product tag | C-His-Avi |
| purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| background | Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. |
| molecular weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| available size | 100 µg, 500 µg |
| endotoxin | Less than 1EU per μg by the LAL method. |
Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Tetramer Protein 3143
$750.00 – $3,000.00
Summary
- Expression: HEK293
- Pure: Yes (HPLC)
- Amino Acid Range: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGAGGVGK peptide
Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Tetramer Protein 3143
| protein |
|---|
| Size and concentration 100, 500µg and lyophilized |
| Form Lyophilized |
| Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Storage buffer Shipped at ambient temperature. |
| Purity > 95% as determined by Tris-Bis PAGE |
| target relevance |
|---|
 |
| The purity of Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Tetramer is greater than 95% as determined by SEC-HPLC. |
 |
| Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
1. **What is the composition of the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143)?**
- The product consists of human leukocyte antigen (HLA) class I molecule HLA-A*1101 (HLA-A*11:01) bound to beta-2 microglobulin (B2M) and a synthetic peptide derived from the KRAS protein with the sequence VVVGAGGVGK.
2. **How should I store the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143)?**
- The tetramer protein should be stored at -80°C upon arrival and can be thawed and refrozen multiple times without loss of activity. It is recommended to aliquot the protein upon receipt to avoid repeated freeze-thaw cycles.
3. **Is the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) compatible with flow cytometry?**
- Yes, this tetramer protein is specifically designed for flow cytometry applications. It can be used to detect and analyze antigen-specific T cells in samples, providing valuable insights into immune responses.
4. **What applications can the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) be used for?**
- This tetramer protein is suitable for various immunological research applications, including flow cytometry analysis of antigen-specific T cells, studying immune responses, and investigating T cell recognition of specific epitopes.
5. **Can the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) be used in vitro cell culture experiments?**
- Yes, this tetramer protein can be used in in vitro cell culture experiments to study antigen-specific T cell responses or to monitor T cell populations. It is important to follow proper cell culture protocols and use appropriate controls for accurate experimental results.
- The product consists of human leukocyte antigen (HLA) class I molecule HLA-A*1101 (HLA-A*11:01) bound to beta-2 microglobulin (B2M) and a synthetic peptide derived from the KRAS protein with the sequence VVVGAGGVGK.
2. **How should I store the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143)?**
- The tetramer protein should be stored at -80°C upon arrival and can be thawed and refrozen multiple times without loss of activity. It is recommended to aliquot the protein upon receipt to avoid repeated freeze-thaw cycles.
3. **Is the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) compatible with flow cytometry?**
- Yes, this tetramer protein is specifically designed for flow cytometry applications. It can be used to detect and analyze antigen-specific T cells in samples, providing valuable insights into immune responses.
4. **What applications can the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) be used for?**
- This tetramer protein is suitable for various immunological research applications, including flow cytometry analysis of antigen-specific T cells, studying immune responses, and investigating T cell recognition of specific epitopes.
5. **Can the Human HLA-A*1101/B2M/KRAS WT VVVGAGGVGK Tetramer Protein (3143) be used in vitro cell culture experiments?**
- Yes, this tetramer protein can be used in in vitro cell culture experiments to study antigen-specific T cell responses or to monitor T cell populations. It is important to follow proper cell culture protocols and use appropriate controls for accurate experimental results.
Publications
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