| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| accession | AAV53343(HLA-A*11:01)&P61769(B2M)&VVGAVGVGK |
| express system | HEK293 |
| product tag | C-His-Avi |
| purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| background | Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail. |
| molecular weight | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Tris-Bis PAGE result. |
| available size | 100 µg, 500 µg |
| endotoxin | Less than 1EU per μg by the LAL method. |
Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein 3178
$310.00 – $1,125.00
Summary
- Expression: HEK293
- Binding assay: Yes (SPR)
- Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAVGVGK peptide
Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein 3178
| protein |
|---|
| Size and concentration 100, 500µg and lyophilized |
| Form Lyophilized |
| Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Storage buffer Shipped at ambient temperature. |
| Purity > 95% as determined by Tris-Bis PAGE |
| target relevance |
|---|
 |
| Western blot analysis of APRIL expression in Jurkat cells with APRIL antibody at 1 µg/mL (A), 2 µg/mL (B), and 4 µg/mL (C). |
 |
| Immunohistochemistry of APRIL in human prostate tissue with APRIL antibody at 10 µg/mL. |
 |
| Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) TCR with an affinity constant of 16.51 µM as determined in SPR assay (Biacore T200). |
 |
| The purity of Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC. |
 |
| Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
1. What are the key features of the Human HLA-A*1101/B2M/KRAS G12V (VVGAVGVGK) Monomer Protein?
- The Human HLA-A*1101/B2M/KRAS G12V Monomer Protein is a recombinant protein that consists of the HLA-A*1101 heavy chain, a beta-2 microglobulin (B2M) light chain, and a KRAS G12V peptide linked together. It is ideal for studying antigen presentation and T cell receptor interactions.
2. How should I store the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein for optimal performance?
- The protein should be stored at -80°C upon arrival. It is recommended to aliquot the protein into smaller portions to avoid repeated freeze-thaw cycles. When handled and stored properly, the protein retains its activity for a long period.
3. Is the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein compatible with specific experimental techniques?
- Yes, this monomer protein is compatible with various techniques commonly used in immunology research, including flow cytometry, cell staining, and T cell functional assays. It can be used to study antigen-specific T cell responses and immune monitoring in cancer research.
4. What are the potential applications of the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein?
- This protein can be used for investigating immune responses to mutant KRAS G12V, a common oncogenic mutation found in cancer. Researchers can use it to study antigen processing and presentation, immune recognition, and T cell activation in the context of cancer immunotherapy.
5. Can the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein be used in combination with other proteins or reagents?
- Yes, this monomer protein can be combined with other proteins, antibodies, or reagents commonly used in immunological assays. Researchers can tailor their experimental setup according to their specific needs and experimental design to investigate immune responses related to KRAS G12V and cancer immunology.
- The Human HLA-A*1101/B2M/KRAS G12V Monomer Protein is a recombinant protein that consists of the HLA-A*1101 heavy chain, a beta-2 microglobulin (B2M) light chain, and a KRAS G12V peptide linked together. It is ideal for studying antigen presentation and T cell receptor interactions.
2. How should I store the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein for optimal performance?
- The protein should be stored at -80°C upon arrival. It is recommended to aliquot the protein into smaller portions to avoid repeated freeze-thaw cycles. When handled and stored properly, the protein retains its activity for a long period.
3. Is the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein compatible with specific experimental techniques?
- Yes, this monomer protein is compatible with various techniques commonly used in immunology research, including flow cytometry, cell staining, and T cell functional assays. It can be used to study antigen-specific T cell responses and immune monitoring in cancer research.
4. What are the potential applications of the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein?
- This protein can be used for investigating immune responses to mutant KRAS G12V, a common oncogenic mutation found in cancer. Researchers can use it to study antigen processing and presentation, immune recognition, and T cell activation in the context of cancer immunotherapy.
5. Can the Human HLA-A*1101/B2M/KRAS G12V Monomer Protein be used in combination with other proteins or reagents?
- Yes, this monomer protein can be combined with other proteins, antibodies, or reagents commonly used in immunological assays. Researchers can tailor their experimental setup according to their specific needs and experimental design to investigate immune responses related to KRAS G12V and cancer immunology.
Publications
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