| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK |
| express system | HEK293 |
| product tag | C-His-Avi |
| purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| background | Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail. |
| molecular weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| available size | 100 µg, 500 µg |
| endotoxin | Less than 1EU per ug by the LAL method. |
Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein 3466
$750.00 – $3,000.00
Summary
- Expression: HEK293
- Functional: Yes (ELISA)
- Amino Acid Range: Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)and VVVGAVGVGK peptide
Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein 3466
| protein |
|---|
| Size and concentration 100, 500µg and lyophilized |
| Form Lyophilized |
| Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Storage buffer Shipped at ambient temperature. |
| Purity > 95% as determined by Tris-Bis PAGE |
| target relevance |
|---|
 |
| Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody captured on CM5 Chip via Protein A can bind Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). |
 |
| Immobilized Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18µg/ml determined by ELISA (QC Test). |
 |
| The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is greater than 95% as determined by SEC-HPLC. |
 |
| Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
1. What is the concentration of the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein?
- The concentration of the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein is provided as 1 mg/mL in a total volume of 100 μL.
2. How should I store this Tetramer Protein for optimal stability?
- To ensure optimal stability, it is recommended to store the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein at -80°C upon arrival. Avoid repeated freeze-thaw cycles to maintain its quality.
3. Is this Tetramer Protein compatible with flow cytometry applications?
- Yes, the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein is compatible with flow cytometry applications for the detection and analysis of T cells specific for the KRAS G12V peptide.
4. Can I use this Tetramer Protein for T cell receptor (TCR) profiling studies?
- Absolutely, this Tetramer Protein is suitable for T cell receptor (TCR) profiling studies, particularly to identify and characterize T cells specific for the KRAS G12V peptide in research settings.
5. Are there any specific references or protocols provided for using this Tetramer Protein?
- Yes, upon purchase, you will receive detailed protocols and references for the proper use and handling of the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein, ensuring accurate and reliable results in your experiments.
- The concentration of the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein is provided as 1 mg/mL in a total volume of 100 μL.
2. How should I store this Tetramer Protein for optimal stability?
- To ensure optimal stability, it is recommended to store the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein at -80°C upon arrival. Avoid repeated freeze-thaw cycles to maintain its quality.
3. Is this Tetramer Protein compatible with flow cytometry applications?
- Yes, the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein is compatible with flow cytometry applications for the detection and analysis of T cells specific for the KRAS G12V peptide.
4. Can I use this Tetramer Protein for T cell receptor (TCR) profiling studies?
- Absolutely, this Tetramer Protein is suitable for T cell receptor (TCR) profiling studies, particularly to identify and characterize T cells specific for the KRAS G12V peptide in research settings.
5. Are there any specific references or protocols provided for using this Tetramer Protein?
- Yes, upon purchase, you will receive detailed protocols and references for the proper use and handling of the Human HLA-A*0301/B2M/KRAS G12V VVVGAVGVL Tetramer Protein, ensuring accurate and reliable results in your experiments.
Publications
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| We haven't added any publications to our database yet. | |||
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