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Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

$750.00$3,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

molecular weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein
Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody captured on Protein A chip, can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).
ELISA with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).
HPLC of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer was greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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