SPR with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

ELISA with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

$750.00 – $3,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide

SKU: 4877parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
molecular weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein 4877

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of Â the MHC Class I, B2M and RMFPNAPYL peptide of Â the WT-1.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

SPR with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein

Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody captured on Protein A chip, can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).

ELISA with Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein

Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).

HPLC of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein

The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.