SPR with Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein 4301

ELISA with Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein 4301

SDS-PAGE gel of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Prot 4301

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein 4301

$310.00 – $1,125.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide

SKU: 4301parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
molecular weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein 4301

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

SPR with Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein

Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 13.43 nM as determined in SPR assay (Biacore T200).

ELISA with Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein

Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test).

HPLC of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein

The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Prot

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.