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Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein 4523

$310.00$1,125.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and YLEPGPVTA peptide of  the gp100.

molecular weight

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein 4523

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

ELISA with Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein
Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
HPLC of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein
The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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