SPR with Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein 3385

ELISA with Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein 3385

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein 3385

$750.00 – $3,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide

SKU: 3385parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
molecular weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein 3385

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

SPR with Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 µM as determined in SPR assay (Biacore T200).

ELISA with Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).

Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag with the EC50 of 26.6ng/ml determined by ELISA.

HPLC of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

Publications

Frequently Asked Questions

1. Question: What is the storage condition for the Human HLA-A*0201/B2M/AFP (FMNKFIYEI) Tetramer Protein?
Answer: The storage condition for this protein is -80°C. It is recommended to aliquot and store the protein at this temperature upon receipt to maintain its stability and functionality.

2. Question: What is the protein concentration of the Human HLA-A*0201/B2M/AFP (FMNKFIYEI) Tetramer Protein?
Answer: The protein concentration of this tetramer protein is 0.5 mg/mL. This concentration is optimized for various applications and experiments.

3. Question: Is this Human HLA-A*0201/B2M/AFP (FMNKFIYEI) Tetramer Protein compatible with flow cytometry applications?
Answer: Yes, this tetramer protein is compatible with flow cytometry applications. It can be used for the detection and analysis of antigen-specific T cells in research studies and immunology experiments.

4. Question: Can this Human HLA-A*0201/B2M/AFP (FMNKFIYEI) Tetramer Protein be used for T cell receptor (TCR) binding assays?
Answer: Yes, this tetramer protein can be utilized for TCR binding assays. It enables the specific detection and characterization of T cell responses targeting the AFP (FMNKFIYEI) peptide.

5. Question: What is the shelf life of the Human HLA-A*0201/B2M/AFP (FMNKFIYEI) Tetramer Protein?
Answer: The shelf life of this protein is 12 months when stored at -80°C. It is important to follow the recommended storage conditions and expiration date for optimal performance and reliability.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.