SPR with Human GITR/TNFRSF18 Protein 4496

ELISA with Human GITR/TNFRSF18 Protein 4496

Human GITR/TNFRSF18 Protein 4496

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln26-Glu161

SKU: 4496parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9Y5U5
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
molecular weight	The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GITR/TNFRSF18 Protein 4496

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Protein names Tumor necrosis factor receptor superfamily member 18 (Activation-inducible TNFR family receptor) (Glucocorticoid-induced TNFR-related protein) (CD antigen CD357)
Gene names TNFRSF18,TNFRSF18 AITR GITR UNQ319/PRO364
Mass 9606Da
Function Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Tissues Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
Structure Binds to TRAF1, TRAF2, and TRAF3, but not TRAF5 and TRAF6. Binds through its C-terminus to SIVA1/SIVA.
Target Relevance information above includes information from UniProt accession: Q9Y5U5
The UniProt Consortium

Data

Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).

Immobilized Human GITR, His Tag at 0.5µg (100µl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test).

Immobilized Human GITR Ligand Trimer, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 0.28µg/ml determined by ELISA.

The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human GITR/TNFRSF18 Protein

Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.