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Human GITR Ligand/TNFSF18 Trimer Protein 4709

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln50-Ser177
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9UNG2

express system

HEK293

product tag

N-His-Flag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

molecular weight

The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human GITR Ligand/TNFSF18 Trimer Protein 4709

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Protein names
Tumor necrosis factor ligand superfamily member 18 (Activation-inducible TNF-related ligand) (AITRL) (Glucocorticoid-induced TNF-related ligand) (hGITRL)
Gene names
TNFSF18,TNFSF18 AITRL GITRL TL6 UNQ149/PRO175
Protein family
Tumor necrosis factor family
Mass
9606Da
Function
FUNCTION: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (PubMed:23892569). Promotes leukocyte adhesion to endothelial cells (PubMed:23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity). {ECO:0000250|UniProtKB:Q7TS55, ECO:0000269|PubMed:17449724, ECO:0000269|PubMed:18040044, ECO:0000269|PubMed:23892569}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17449724}; Single-pass type II membrane protein {ECO:0000269|PubMed:17449724}.
Tissues
TISSUE SPECIFICITY: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
Structure
SUBUNIT: Homodimer (By similarity). Homotrimer. {ECO:0000250, ECO:0000269|PubMed:18040044}.
Target Relevance information above includes information from UniProt accession : Q9UNG2
The UniProt Consortium

Data

ELISA with Human GITR Ligand/TNFSF18 Trimer Protein
Immobilized Human GITR Ligand Trimer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44µg/ml determined by ELISA.
HPLC of Human GITR Ligand/TNFSF18 Trimer Protein
The purity of Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human GITR Ligand/TNFSF18 Trimer Protein
Human GITR Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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