ELISA with Human GFR alpha 1 Protein 4134

Human GFR alpha 1 Protein 4134

$210.00 – $700.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp25-Ser424

SKU: 4134parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_665736
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
molecular weight	The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GFR alpha 1 Protein 4134

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
Protein names GDNF family receptor alpha-1 (GDNF receptor alpha-1) (GDNFR-alpha-1) (GFR-alpha-1) (RET ligand 1) (TGF-beta-related neurotrophic factor receptor 1)
Protein family GDNFR family
Mass 51456Da
Function Coreceptor for GDNF, a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed:10829012, PubMed:31535977). GDNF-binding leads to autophosphorylation and activation of the RET receptor (PubMed:31535977). {ECO:0000269\|PubMed:10829012, ECO:0000269\|PubMed:31535977}.
Subellular location Cell membrane {ECO:0000250\|UniProtKB:Q62997}; Lipid-anchor, GPI-anchor {ECO:0000250\|UniProtKB:Q62997}. Golgi apparatus, trans-Golgi network {ECO:0000250\|UniProtKB:Q62997}. Endosome {ECO:0000250\|UniProtKB:Q62997}. Endosome, multivesicular body {ECO:0000250\|UniProtKB:Q62997}. Note=Localizes mainly to the plasma membrane. In the presence of SORL1, shifts to vesicular structures, including trans-Golgi network, endosomes and multivesicular bodies. {ECO:0000250\|UniProtKB:Q62997}.
Structure Interacts with GDNF ligand and RET: forms a 2:2:2 ternary complex composed of GDNF ligand, GFRA1 and RET receptor (PubMed:23333276, PubMed:31535977). Interacts with SORL1, either alone or in complex with GDNF (PubMed:23333276). Interaction between SORL1 and GFRA1 leads to GFRA1 internalization, but not degradation (PubMed:23333276). {ECO:0000269\|PubMed:23333276, ECO:0000269\|PubMed:31535977}.
Target Relevance information above includes information from UniProt accession: P56159
The UniProt Consortium

Data

Immobilized Human GDNF, No Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 5.0ng/ml determined by ELISA.

The purity of Human GFR alpha 1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human GFR alpha 1 Protein

Human GFR alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.