ELISA with Human GARP (G139N) &Latent TGF Beta 1 Complex Protein 3532

Human GARP (G139N) &Latent TGF Beta 1 Complex Protein 3532

$450.00 – $1,500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: His20-Leu628(GARP(G139N)) & Leu30-Ser390(Latent TGF beta 1)

SKU: 3532parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q14392(GARP(G139N))&P01137(Latent TGF beta 1)
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
molecular weight	The protein has a predicted MW of 70.3 kDa (GARP(G139N))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 75-80 kDa (GARP(G139N))&15 kDa&42-48 kDa (Latent TGF Beta 1) based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GARP (G139N) &Latent TGF Beta 1 Complex Protein 3532

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Protein names Transforming growth factor beta activator LRRC32 (Garpin) (Glycoprotein A repetitions predominant) (GARP) (Leucine-rich repeat-containing protein 32)
Gene names LRRC32,LRRC32 D11S833E
Protein family LRRC32/LRRC33 family
Mass 71979Da
Function Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:19651619, PubMed:19750484, PubMed:22278742). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:22278742). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed:22278742). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed:19651619, PubMed:19750484). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).
Subellular location Cell membrane ; Single-pass type I membrane protein. Cell surface .
Tissues Preferentially expressed in regulatory T-cells (Tregs).
Structure Interacts with TGFB1; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1, leading to regulate activation of TGF-beta-1 (PubMed:19651619, PubMed:22278742). Interacts with TGFB2 (PubMed:19651619). Interacts with TGFB3; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB3, leading to regulate activation of TGF-beta-3 (By similarity). Interacts with LAPTM4B; decreases TGFB1 production in regulatory T-cells (PubMed:26126825).
Target Relevance information above includes information from UniProt accession: Q14392
The UniProt Consortium

Data

ELISA with Human GARP (G139N) &Latent TGF Beta 1 Complex Protein

Immobilized Human GARP (G139N) &Latent TGF Beta 1 Complex, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 0.73µg/ml determined by ELISA.

HPLC of Human GARP (G139N) &Latent TGF Beta 1 Complex Protein

The purity of Human GARP (G139N) &Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human GARP (G139N) &Latent TGF Beta 1 Complex Protein

Human GARP (G139N) &Latent TGF Beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Database connection successful.

Frequently Asked Questions

1. Q: What are the features of the Human GARP (G139N) & Latent TGF-beta 1 Complex Protein (3532)?

A: The Human GARP (G139N) & Latent TGF-beta 1 Complex Protein (3532) is a high-quality protein complex that consists of GARP (glycoprotein A repetitions predominant) and latent transforming growth factor beta 1 (TGF-beta 1). This complex plays a crucial role in regulating immune responses and TGF-beta activation.

2. Q: How should this protein complex be stored?

A: This protein complex should be stored at -80°C upon arrival. It is recommended to aliquot the product into smaller quantities to avoid repeated freeze-thaw cycles, which can affect its stability and activity.

3. Q: Is this Human GARP & Latent TGF-beta 1 Complex Protein compatible with specific research applications?

A: Yes, this protein complex is compatible with various research applications, including functional assays, cell culture studies, and protein-protein interaction studies related to immune regulation, TGF-beta signaling, and inflammation research.

4. Q: Can this product be used for in vivo studies or only in vitro experiments?

A: While this protein complex is primarily designed for in vitro research experiments and functional assays, it may also be suitable for some in vivo studies, provided proper validation and experimental conditions are met.

5. Q: Are there any recommended references or protocols available for using this Human GARP & Latent TGF-beta 1 Complex Protein in research?

A: Yes, the product page provides relevant references and protocols to guide researchers on the applications, experimental design, and data interpretation involving this protein complex. Additionally, our technical support team is available to assist with any specific queries or troubleshooting related to using this product in your research.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

We havent added any publications to our database yet.