ELISA with Human FCRL2/IRTA4 Protein 3451

Human FCRL2/IRTA4 Protein 3451

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu20-Val401

SKU: 3451parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q96LA5
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation; P < 0.0001) and cells of other hematological diseases.
molecular weight	The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human FCRL2/IRTA4 Protein 3451

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation; P < 0.0001) and cells of other hematological diseases.
Protein names Fc receptor-like protein 2 (FcR-like protein 2) (FcRL2) (Fc receptor homolog 2) (FcRH2) (IFGP family protein 4) (Immunoglobulin receptor translocation-associated protein 4) (SH2 domain-containing phosphatase anchor protein 1) (CD antigen CD307b)
Gene names FCRL2,FCRL2 FCRH2 IFGP4 IRTA4 SPAP1 UNQ9236/PRO31998
Mass 9606Da
Function May have an regulatory role in normal and neoplastic B cell development.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed in the secondary lymphoid organs, spleen and lymph node. Expression is limited to the mature B-cell lines. Highly expressed in CD19 and within the mantle zones of the tonsil tissue. Isoform 2 is expressed in the spleen, peripheral blood and bone marrow. Isoform 2 and isoform 4 are expressed in B-cell lines. Preferentially expressed in memory B-cells (at protein level).
Structure The tyrosine-phosphorylated isoform 2 interacts with PTPN6.
Post-translational modification Isoform 2 is N- and O-glycosylated, and phosphorylated.
Domain Co
Target Relevance information above includes information from UniProt accession: Q96LA5
The UniProt Consortium

Data

Immobilized Human FCRL2, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-FCRL2 Antibody, hFc Tag with the EC50 of 0.13µg/ml determined by ELISA.

The purity of Human FCRL2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human FCRL2/IRTA4 Protein

Human FCRL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.