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Human Fc gamma RIIIA/CD16a (V176) Protein 4516

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly17-Gln208(V176)
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

AAH17865

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

molecular weight

The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Fc gamma RIIIA/CD16a (V176) Protein 4516

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Human Fc gamma RIIIA/CD16a (V176) Protein
Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).
ELISA with Human Fc gamma RIIIA/CD16a (V176) Protein
Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 23.3ng/ml determined by ELISA (QC test).
HPLC of Human Fc gamma RIIIA/CD16a (V176) Protein
The purity of Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Fc gamma RIIIA/CD16a (V176) Protein
Human Fc gamma RIIIA (V176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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