SPR with Human Fc gamma RIIB/CD32b Protein 4500

Human Fc gamma RIIB/CD32b Protein 4500

$240.00 – $800.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Ala46-Pro217

SKU: 4500parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P31994
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
molecular weight	The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Fc gamma RIIB/CD32b Protein 4500

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Protein names Low affinity immunoglobulin gamma Fc region receptor II-b (IgG Fc receptor II-b) (CDw32) (Fc-gamma RII-b) (Fc-gamma-RIIb) (FcRII-b) (CD antigen CD32)
Mass 34044Da
Function Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Subellular location Cell membrane; Single-pass type I membrane protein.
Tissues Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
Structure Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN. {ECO:0000269\|PubMed:8327512, ECO:0000269\|PubMed:9232445}.; (Microbial infection) Isoform IIB1 interacts with measles virus protein N. Protein N is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. {ECO:0000269\|PubMed:15914856}.
Post-translational modification Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. {ECO:0000269\|PubMed:8756631}.
Domain Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-ba
Target Relevance information above includes information from UniProt accession: P31994
The UniProt Consortium

Data

SPR with Human Fc gamma RIIB/CD32b Protein

Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB, His Tag with an affinity constant of 3.46 µM as determined in SPR assay (Biacore T200).

HPLC of Human Fc gamma RIIB/CD32b Protein

The purity of Human Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Fc gamma RIIB/CD32b Protein

Human Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.