SPR with Human Fc gamma RIIA/CD32a (H167) Protein 4554

Human Fc gamma RIIA/CD32a (H167) Protein 4554

$240.00 – $800.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Ala36-Ile218(H167)

SKU: 4554parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P12318
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
molecular weight	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Fc gamma RIIA/CD32a (H167) Protein 4554

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Protein names Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a) (CDw32) (Fc-gamma RII-a) (Fc-gamma-RIIa) (FcRII-a) (CD antigen CD32)
Gene names FCGR2A,FCGR2A CD32 FCG2 FCGR2A1 IGFR2
Mass 9606Da
Function Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Found on monocytes, neutrophils and eosinophil platelets.
Structure Interacts with IGHG1 (PubMed:11711607). Interacts with INPP5D/SHIP1 and INPPL1/SHIP2, regulating its function. Interacts with APCS and FGR. Interacts with HCK.
Post-translational modification Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Target Relevance information above includes information from UniProt accession: P12318
The UniProt Consortium

Data

SPR with Human Fc gamma RIIA/CD32a (H167) Protein

Human Fc gamma RIIA (H167) , His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 1.53 µM as determined in SPR assay (Biacore T200) (QC Test).

Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His Tag with an affinity constant of 0.73 µM as determined in SPR assay (Biacore T200).

HPLC of Human Fc gamma RIIA/CD32a (H167) Protein

The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Fc gamma RIIA/CD32a (H167) Protein

Human Fc gamma RIIA (H167) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.