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Human Fc gamma RI/CD64 Protein 4556

$240.00$800.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Gln16-Pro288
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P12314

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE

background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

molecular weight

The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Fc gamma RI/CD64 Protein 4556

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Protein names
High affinity immunoglobulin gamma Fc receptor I (IgG Fc receptor I) (Fc-gamma RI) (FcRI) (Fc-gamma RIA) (FcgammaRIa) (CD antigen CD64)
Gene names
FCGR1A,FCGR1A FCG1 FCGR1 IGFR1
Protein family
Immunoglobulin superfamily, FCGR1 family
Mass
9606Da
Function
FUNCTION: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells. {ECO:0000269|PubMed:10397749, ECO:0000269|PubMed:10514529, ECO:0000269|PubMed:11711607, ECO:0000269|PubMed:21965667, ECO:0000269|PubMed:8611682, ECO:0000269|PubMed:9881690}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12756162, ECO:0000269|PubMed:8611682}; Single-pass type I membrane protein {ECO:0000269|PubMed:12756162, ECO:0000269|PubMed:8611682}. Note=Stabilized at the cell membrane through interaction with FCER1G.
Tissues
TISSUE SPECIFICITY: Monocyte/macrophage specific.
Structure
SUBUNIT: Interacts with IGHG1 (PubMed:11711607). Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN. {ECO:0000269|PubMed:10514529, ECO:0000269|PubMed:10781611, ECO:0000269|PubMed:11711607, ECO:0000269|PubMed:15229321, ECO:0000269|PubMed:18023480, ECO:0000269|PubMed:18322202, ECO:0000269|PubMed:8064233, ECO:0000269|PubMed:8611682}.
Post-translational modification
PTM: Phosphorylated on serine residues. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession : P12314
The UniProt Consortium

Data

SPR with Human Fc gamma RI/CD64 Protein
Human Fc gamma RI, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 5.25 nM as determined in SPR assay (Biacore T200).
SDS-PAGE gel of Human Fc gamma RI/CD64 Protein
Human Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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