SPR with Human Ephrin-B2/EFNB2 Protein 4200

ELISA with Human Ephrin-B2/EFNB2 Protein 4200

Human Ephrin-B2/EFNB2 Protein 4200

$135.00 – $450.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile28-Ala229

SKU: 4200parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_004084
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
molecular weight	The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Ephrin-B2/EFNB2 Protein 4200

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
Protein names Ephrin-B2 (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L)
Protein family Ephrin family
Mass 36923Da
Function Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. {ECO:0000269\|PubMed:12734395}.; (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. {ECO:0000269\|PubMed:15998730, ECO:0000269\|PubMed:16007075, ECO:0000269\|PubMed:16477309, ECO:0000269\|PubMed:17376907}.
Subellular location Cell membrane {ECO:0000269\|PubMed:28931592}; Single-pass type I membrane protein {ECO:0000255}. Cell junction, adherens junction {ECO:0000250\|UniProtKB:P52800}.
Tissues Lung and kidney.
Structure Interacts with PDZRN3 (By similarity). Binds to the receptor tyrosine kinases EPHA4, EPHB4 and EPHA3. {ECO:0000250, ECO:0000269\|PubMed:16867992, ECO:0000269\|PubMed:19836338, ECO:0000269\|PubMed:19875447}.; (Microbial infection) Interacts with Hendra virus and Nipah virus G protein (PubMed:15998730, PubMed:16007075, PubMed:16477309, PubMed:17376907). {ECO:0000269\|PubMed:15998730, ECO:0000269\|PubMed:16007075, ECO:0000269\|PubMed:16477309, ECO:0000269\|PubMed:17376907}.
Post-translational modification Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: P52799
The UniProt Consortium

Data

Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).

ELISA with Human Ephrin-B2/EFNB2 Protein

Immobilized Human EPHB2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).

The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Ephrin-B2/EFNB2 Protein

Human Ephrin B2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.