Cell based assay with Human EGF Protein 3126

Human EGF Protein 3126

$270.00 – $900.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Asn971-Arg1023

SKU: 3126parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P01133
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
molecular weight	The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human EGF Protein 3126

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Protein names Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]
Gene names EGF,EGF
Mass 9606Da
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed in kidney, salivary gland, cerebrum and prostate.
Structure Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2 (By similarity). Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD).
Post-translational modification O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Target Relevance information above includes information from UniProt accession: P01133
The UniProt Consortium

Data

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human EGFR, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate(5µg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL.

The purity of Human EGF is greater than 95% as determined by SEC-HPLC.

Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.