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Human E-Selectin/CD62E Protein 4615

$150.00$500.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Trp22-Pro556
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P16581

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 – 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

molecular weight

The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human E-Selectin/CD62E Protein 4615

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein names
E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
Gene names
SELE,SELE ELAM1
Protein family
Selectin/LECAM family
Mass
9606Da
Function
FUNCTION: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. {ECO:0000269|PubMed:1689848, ECO:0000269|PubMed:28011641}.
Catalytic activity
BINDING 101..109; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 101; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 103; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 109; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 113..118; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 126..128; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 126; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"; BINDING 127; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY"
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12649084, ECO:0000269|PubMed:28011641}; Single-pass type I membrane protein.
Structure
SUBUNIT: Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction. {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:18606703}.
Target Relevance information above includes information from UniProt accession : P16581
The UniProt Consortium

Data

ELISA with Human E-Selectin/CD62E Protein
Immobilized Human E-Selectin, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
HPLC of Human E-Selectin/CD62E Protein
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human E-Selectin/CD62E Protein
Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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