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Human/Cynomolgus/Rhesus macaque CD28 Protein 4476

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Asn19-Pro152
SKU: 4476parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P10747

express system

HEK293

product tag

N-His-Avi

purity

> 95% as determined by Tris-Bis PAGE

background

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

molecular weight

The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human/Cynomolgus/Rhesus macaque CD28 Protein 4476

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Protein names
T-cell-specific surface glycoprotein CD28 (TP44) (CD antigen CD28)
Gene names
CD28,CD28
Mass
9606Da
Function
FUNCTION: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). {ECO:0000269|PubMed:15067037, ECO:0000269|PubMed:8617933}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Cell surface {ECO:0000269|PubMed:15067037}.
Tissues
TISSUE SPECIFICITY: Expressed in T-cells and plasma cells, but not in less mature B-cells.
Structure
SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2. Isoform 3 interacts with CD40LG (PubMed:15067037). {ECO:0000269|PubMed:15067037, ECO:0000269|PubMed:15696168, ECO:0000269|PubMed:24098653}.
Post-translational modification
PTM: CD40LG induces tyrosine phosphorylation of isoform 3. {ECO:0000269|PubMed:15067037}.
Target Relevance information above includes information from UniProt accession : P10747
The UniProt Consortium

Data

SPR with Human/Cynomolgus/Rhesus macaque CD28 Protein
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 µM as determined in SPR assay (Biacore T200).
SPR with Human/Cynomolgus/Rhesus macaque CD28 Protein
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).
ELISA with Human/Cynomolgus/Rhesus macaque CD28 Protein
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
SDS-PAGE gel of Human/Cynomolgus/Rhesus macaque CD28 Protein
Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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