ELISA with Human/Cynomolgus/Rhesus macaque CD28 Protein 4184

Human/Cynomolgus/Rhesus macaque CD28 Protein 4184

$150.00 – $500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asn19-Pro152

SKU: 4184parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P10747
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
molecular weight	The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human/Cynomolgus/Rhesus macaque CD28 Protein 4184

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Protein names T-cell-specific surface glycoprotein CD28 (TP44) (CD antigen CD28)
Gene names CD28,CD28
Mass 9606Da
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Subellular location Membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell surface .
Tissues Expressed in T-cells and plasma cells, but not in less mature B-cells.
Structure Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2. Isoform 3 interacts with CD40LG (PubMed:15067037).
Post-translational modification CD40LG induces tyrosine phosphorylation of isoform 3.
Target Relevance information above includes information from UniProt accession: P10747
The UniProt Consortium

Data

ELISA with Human/Cynomolgus/Rhesus macaque CD28 Protein

Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 46.3ng/ml determined by ELISA.

HPLC of Human/Cynomolgus/Rhesus macaque CD28 Protein

The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human/Cynomolgus/Rhesus macaque CD28 Protein

Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.