Human CXCL13/BCA-1 Protein 5099

$300.00 – $1,000.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Val23-Pro109

SKU: 5099parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O43927
express system	E.coli
product tag	N-His-Sumo
purity	> 95% as determined by Tris-Bis PAGE
background	Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.
molecular weight	The protein has a predicted MW of 22.9 kDa. The protein migrates to 26-28 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CXCL13/BCA-1 Protein 5099

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.
Protein names C-X-C motif chemokine 13 (Angie) (B cell-attracting chemokine 1) (BCA-1) (B lymphocyte chemoattractant) (CXC chemokine BLC) (Small-inducible cytokine B13)
Gene names CXCL13,CXCL13 BCA1 BLC SCYB13
Protein family Intercrine alpha (chemokine CxC) family
Mass 9606Da
Function Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
Subellular location Secreted.
Tissues Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
Target Relevance information above includes information from UniProt accession: O43927
The UniProt Consortium

Data

Immobilized Anti-CXCL13 Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human CXCL13, His Tag with the EC50 of 0.54µg/ml determined by ELISA.

SDS-PAGE gel of Human CXCL13/BCA-1 Protein

Human CXCL13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.