Human CX3CL1/Fractalkine Protein 4280

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Gln25-Gln341

SKU: 4280parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AAH01163
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as pancreatic ductal adenocarcinoma.
molecular weight	The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CX3CL1/Fractalkine Protein 4280

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as pancreatic ductal adenocarcinoma.
Protein names Fractalkine (FK) (ABCD-3) (C-X3-C motif chemokine 1) (CX3C membrane-anchored chemokine) (Neurotactin) (Small-inducible cytokine D1) [Cleaved into: Processed fractalkine]
Gene names Cx3cl1,Cx3cl1 Cx3c Fkn Scyd1
Protein family Intercrine delta family
Mass 10090Da
Function Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:10187784, PubMed:18971423). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:10187784, PubMed:10382755, PubMed:18971423, PubMed:9177350). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:10382755, PubMed:9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (By similarity). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (By similarity). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (By similarity).; [Processed fractalkine]: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.; [Fractalkine]: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.
Subellular location Cell membrane ; Single-pass type I membrane protein .; [Processed fractalkine]: Secreted .
Tissues Highest levels in brain (PubMed:10508268, PubMed:9177350, PubMed:9479488, PubMed:9845323). Lower levels in kidney, heart and lung (PubMed:10508268, PubMed:9177350, PubMed:9479488). Also found in skeletal muscle and testis (PubMed:9177350, PubMed:9479488). Highly expressed in lesional smooth muscle cells, but not macrophages (PubMed:12569158). Low levels of ABCD-3 mRNA were also found in anti-CD40-stimulated splenic B-cells, but not in resting B-cells. Also expressed in dendritic cells (PubMed:10508268).
Structure Monomer (By similarity). Forms a ternary complex with CX3CR1 and ITGAV:ITGB3 or ITGA4:ITGB1 (By similarity).
Post-translational modification A soluble short 80 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
Target Relevance information above includes information from UniProt accession: O35188
The UniProt Consortium

Data

HPLC of Human CX3CL1/Fractalkine Protein

The purity of Human CX3CL1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CX3CL1/Fractalkine Protein

Human CX3CL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.