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Human CLEC12A/MICL/CLL-1 Protein 4889

$345.00$1,150.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: His65-Ala265
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q5QGZ9

express system

HEK293

product tag

N-His

purity

> 95% as determined by Tris-Bis PAGE

background

CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.

molecular weight

The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CLEC12A/MICL/CLL-1 Protein 4889

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Protein names
C-type lectin domain family 12 member A (C-type lectin-like molecule 1) (CLL-1) (Dendritic cell-associated lectin 2) (DCAL-2) (Myeloid inhibitory C-type lectin-like receptor) (MICL) (CD antigen CD371)
Gene names
CLEC12A,CLEC12A CLL1 DCAL2 MICL
Mass
9606Da
Function
FUNCTION: Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. {ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:16239426}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:15548716, ECO:0000269|PubMed:16239426, ECO:0000269|PubMed:16838277}; Single-pass type II membrane protein {ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:15548716, ECO:0000269|PubMed:16239426, ECO:0000269|PubMed:16838277}. Note=Ligand binding leads to internalization.
Tissues
TISSUE SPECIFICITY: Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages. {ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:15548716, ECO:0000269|PubMed:16239426, ECO:0000269|PubMed:16838277}.
Structure
SUBUNIT: Interacts with PTPN6 and PTPN11. {ECO:0000269|PubMed:14739280}.
Post-translational modification
PTM: Highly N-glycosylated. Glycosylation varies between cell types. {ECO:0000269|PubMed:14739280, ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:16838277}.
Domain
DOMAIN: Co
Target Relevance information above includes information from UniProt accession : Q5QGZ9
The UniProt Consortium

Data

ELISA with Human CLEC12A/MICL/CLL-1 Protein
Immobilized Human CLEC12A at 0.5µg (100µl/Well) on the plate. Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
SDS-PAGE gel of Human CLEC12A/MICL/CLL-1 Protein
Human CLEC12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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