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Human CLEC12A/MICL/CLL-1 Protein 4402

$345.00$1,150.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: His65-Ala265
SKU: 4402parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q5QGZ9

express system

HEK293

product tag

N-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.

molecular weight

The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 68-73 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CLEC12A/MICL/CLL-1 Protein 4402

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Protein names
C-type lectin domain family 12 member A (C-type lectin-like molecule 1) (CLL-1) (Dendritic cell-associated lectin 2) (DCAL-2) (Myeloid inhibitory C-type lectin-like receptor) (MICL) (CD antigen CD371)
Gene names
CLEC12A,CLEC12A CLL1 DCAL2 MICL
Mass
9606Da
Function
Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.
Subellular location
Cell membrane ; Single-pass type II membrane protein. Note=Ligand binding leads to internalization.
Tissues
Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages.
Structure
Interacts with PTPN6 and PTPN11.
Post-translational modification
Highly N-glycosylated. Glycosylation varies between cell types.
Domain
Co
Target Relevance information above includes information from UniProt accession: Q5QGZ9
The UniProt Consortium

Data

ELISA with Human CLEC12A/MICL/CLL-1 Protein
Immobilized Human CLEC12A, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC12A Antibody, hFc Tag with the EC50 of 20.2ng/ml determined by ELISA.
HPLC of Human CLEC12A/MICL/CLL-1 Protein
The purity of Human CLEC12A is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CLEC12A/MICL/CLL-1 Protein
Human CLEC12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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