Human CD6 Protein 3082

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: His18-Leu402

SKU: 3082parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_006716.3
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
molecular weight	The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD6 Protein 3082

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
Protein names T-cell differentiation antigen CD6 (T12) (TP120) (CD antigen CD6) [Cleaved into: Soluble CD6]
Mass 71801Da
Function Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24584089, PubMed:24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777). {ECO:0000269\|PubMed:15048703, ECO:0000269\|PubMed:15294938, ECO:0000269\|PubMed:16352806, ECO:0000269\|PubMed:16914752, ECO:0000269\|PubMed:17601777, ECO:0000269\|PubMed:24584089, ECO:0000269\|PubMed:24945728}.
Subellular location Cell membrane {ECO:0000269\|PubMed:15048703, ECO:0000269\|PubMed:15294938, ECO:0000269\|PubMed:16352806, ECO:0000269\|PubMed:16914752, ECO:0000269\|PubMed:17371992, ECO:0000269\|PubMed:17601777, ECO:0000269\|PubMed:24945728, ECO:0000269\|PubMed:9013954}; Single-pass type I membrane protein {ECO:0000269\|PubMed:17371992}. Note=Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with the TCR/CD3 complex at the immunological synapse (PubMed:15294938). {ECO:0000269\|PubMed:15294938}.; [Soluble CD6]: Secreted {ECO:0000269\|PubMed:17601777}. Note=The origins of the secreted form are not clear, but it might be created by proteolytic shedding of the ectodomain. {ECO:0000305}.
Tissues Detected on thymocytes (PubMed:15294938). Detected on peripheral blood T-cells (PubMed:15048703, PubMed:16352806). Detected on natural killer (NK) cells (PubMed:16352806). Soluble CD6 is detected in blood serum (at protein level) (PubMed:17601777). Detected in spleen, thymus, appendix, lymph node and peripheral blood leukocytes (PubMed:9013954). Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. {ECO:0000269\|PubMed:15048703, ECO:0000269\|PubMed:15294938, ECO:0000269\|PubMed:16352806, ECO:0000269\|PubMed:17601777, ECO:0000269\|PubMed:9013954}.
Structure Interacts (via extracellular domain) with ALCAM/CD166 (via extracellular domain) (PubMed:15048703, PubMed:16352806, PubMed:16914752, PubMed:24945728, PubMed:26146185). Interacts with the TCR/CD3 complex subunit CD3E (PubMed:15294938). Interacts (via tyrosine phosphorylated C-terminus) with LCP2 (via SH2 domain) (PubMed:16914752). Interacts with VAV1 (By similarity). Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3 (PubMed:24945728). Interaction with LGALS1 or LGALS3 inhibits interaction with ALCAM (PubMed:24945728). {ECO:0000250\|UniProtKB:Q61003, ECO:0000269\|PubMed:15048703, ECO:0000269\|PubMed:15294938, ECO:0000269\|PubMed:16352806, ECO:0000269\|PubMed:16914752, ECO:0000269\|PubMed:24945728, ECO:0000269\|PubMed:26146185}.
Post-translational modification After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues. {ECO:0000269\|PubMed:16914752, ECO:0000269\|PubMed:2016320, ECO:0000269\|PubMed:24584089, ECO:0000269\|PubMed:7678115}.; Glycosylated. {ECO:0000305\|PubMed:24945728}.
Target Relevance information above includes information from UniProt accession: P30203
The UniProt Consortium

Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 µM as determined in SPR assay (Biacore T200).

Immobilized Human CD6, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).

The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.

Human CD6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Frequently Asked Questions

1. What is the shelf life of the Human CD6 Protein (3082) offered on benchmarkantibodies.com?
Answer: The Human CD6 Protein (3082) has a shelf life of 24 months when stored at -70°C upon arrival.

2. Can the Human CD6 Protein (3082) be used for functional studies or assays?
Answer: Yes, the Human CD6 Protein (3082) can be used for functional studies and assays related to its cell adhesion and co-stimulation properties.

3. Is this Human CD6 Protein (3082) compatible with a wide range of applications?
Answer: Yes, the Human CD6 Protein (3082) is suitable for various applications, including ELISA, western blotting, and flow cytometry.

4. How should I store the Human CD6 Protein (3082) once I receive it?
Answer: Upon arrival, it is recommended to store the Human CD6 Protein (3082) at -70°C to maintain its stability and functionality.

5. Does the Human CD6 Protein (3082) come with any additional documentation or quality control information?
Answer: Yes, the Human CD6 Protein (3082) is provided with detailed product specifications, quality control data, and instructions for use to ensure optimal results in experimental settings.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.