ELISA with Human CD40 Ligand/TNFSF5 Trimer Protein 4450

Binding assay with Human CD40 Ligand/TNFSF5 Trimer Protein 4450

Human CD40 Ligand/TNFSF5 Trimer Protein 4450

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (binding assay)
Amino Acid Range: Met113-Leu261

SKU: 4450parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P29965
express system	HEK293
product tag	N-monomeric hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. .
molecular weight	The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD40 Ligand/TNFSF5 Trimer Protein 4450

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD40 ligand or CD40L, also called CD154,Â is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.Â .
Protein names CD40 ligand (CD40-L) (T-cell antigen Gp39) (TNF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD antigen CD154) [Cleaved into: CD40 ligand, membrane form; CD40 ligand, soluble form (sCD40L)]
Gene names CD40LG,CD40LG CD40L TNFSF5 TRAP
Protein family Tumor necrosis factor family
Mass 9606Da
Function Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).; [CD40 ligand, soluble form]: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Subellular location Cell membrane ; Single-pass type II membrane protein. Cell surface .; [CD40 ligand, soluble form]: Secreted. Note=Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissues Specifically expressed on activated CD4+ T-lymphocytes.
Structure Homotrimer (PubMed:11676606, PubMed:8589998, PubMed:8626375). Interacts with isoform 3 of CD28 (PubMed:15067037). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (PubMed:31331973, PubMed:8626375). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (PubMed:31331973).
Post-translational modification The soluble form derives from the membrane form by proteolytic processing.; N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.; Not O-glycosylated.
Target Relevance information above includes information from UniProt accession: P29965
The UniProt Consortium

Data

ELISA with Human CD40 Ligand/TNFSF5 Trimer Protein

Immobilized Human CD40, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).

Binding assay with Human CD40 Ligand/TNFSF5 Trimer Protein

Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.

HPLC of Human CD40 Ligand/TNFSF5 Trimer Protein

The purity of Human CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CD40 Ligand/TNFSF5 Trimer Protein

Human CD40 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.