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Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein 4423

$330.00$1,100.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P07766(CD3E)&P04234(CD3D)

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

molecular weight

The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-58 kDa (CD3E) and 42-48 kDa (CD3D) based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein 4423

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

ELISA with Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
Immobilized Human CD3E&CD3D, hFc Tag at 0.5µg/ml (100µl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA.
ELISA with Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
Immobilized Human CD3E&CD3D, hFc Tag at 1µg/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 45.9ng/ml determined by ELISA (QC Test).
ELISA with Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
Immobilized Tebentafusp, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
HPLC of Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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