SPR with Human CD28H/IGPR-1 Protein 4143

ELISA with Human CD28H/IGPR-1 Protein 4143

Human CD28H/IGPR-1 Protein 4143

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu23-Gly150

SKU: 4143parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q96BF3
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
molecular weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD28H/IGPR-1 Protein 4143

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
Protein names Transmembrane and immunoglobulin domain-containing protein 2 (CD28 homolog) (Immunoglobulin and proline-rich receptor 1) (IGPR-1)
Gene names TMIGD2,TMIGD2 CD28H IGPR1 UNQ3059/PRO9879
Mass 9606Da
Function Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Widely expressed, mainly by epithelial and endothelial cells, including bronchial epithelial cells of lung, breast glandular and lobular epithelia cells, urothelium of the bladder, skin epidermis, epithelium of gastrointestinal, rectum, endometrial glands of the uterus, ureter, fallopian tube epithelium, colonic epithelium, small bowl epithelium, stomach epithelium, including both chief and parietal cells, trophoblastic epithelium of placenta, and pancreatic acinar cells (at protein level). Consistently expressed in veins and arteries (at protein level). Not detected in thyroid, cerebellum, cerebral cortex and thymus (at protein level). Expressed in lymphoid organs, with highest levels in thymus, spleen, peripheral blood lymphocytes and liver. In the thymus, expressed in CD4+ and CD8+ single- and double-positive cells, but not in immature CD4- and CD8- double-negative cells (at protein level). In peripheral blood mononuclear cells, highly expressed on CD56+ or CD16+ natural killer cells and CD3+ T-cells(at protein level). Not detected on B-cells(at protein level). Expressed in tonsils (at protein level).
Structure May form homophilic interactions that could regulate cell-cell interaction. Interacts with CACNB2, DST, MIA and NCKIPSD. Interacts with HHLA2.
Post-translational modification N-glycosylated.
Target Relevance information above includes information from UniProt accession: Q96BF3
The UniProt Consortium

Data

Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).

Immobilized Human B7-H7, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.89µg/ml determined by ELISA (QC Test).

The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CD28H/IGPR-1 Protein

Human CD28H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.