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Human CD27 Ligand/CD70 Trimer Protein 5213

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Leu50-Pro193
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P32970

express system

HEK293

product tag

N-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

molecular weight

The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-65 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD27 Ligand/CD70 Trimer Protein 5213

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Protein names
CD70 antigen (CD27 ligand) (CD27-L) (Tumor necrosis factor ligand superfamily member 7) (CD antigen CD70)
Gene names
CD70,CD70 CD27L CD27LG TNFSF7
Protein family
Tumor necrosis factor family
Mass
9606Da
Function
FUNCTION: Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864). {ECO:0000269|PubMed:28011863, ECO:0000269|PubMed:28011864, ECO:0000269|PubMed:8387892}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11920585, ECO:0000269|PubMed:28011863, ECO:0000269|PubMed:28011864, ECO:0000269|PubMed:8120384, ECO:0000269|PubMed:8387892}; Single-pass type II membrane protein {ECO:0000255}.
Structure
SUBUNIT: Homotrimer. {ECO:0000269|PubMed:34419446}.
Post-translational modification
PTM: N-glycosylated. {ECO:0000269|PubMed:34419446}.
Target Relevance information above includes information from UniProt accession : P32970
The UniProt Consortium

Data

ELISA with Human CD27 Ligand/CD70 Trimer Protein
Immobilized Human CD27 Ligand Trimer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
HPLC of Human CD27 Ligand/CD70 Trimer Protein
The purity of Human CD27 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CD27 Ligand/CD70 Trimer Protein
Human CD27 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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