Human CCL5 (Rantes) Protein 3881

$300.00 – $1,000.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Ser24-Ser91

SKU: 3881parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P13501
express system	E.coli
product tag	N-His, SUMO
purity	> 95% as determined by Tris-Bis PAGE
background	The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
molecular weight	The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CCL5 (Rantes) Protein 3881

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
Protein names C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68); RANTES(4-68)]
Gene names CCL5,CCL5 D17S136E SCYA5
Protein family Intercrine beta (chemokine CC) family
Mass 9990Da
Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Subellular location Secreted.
Tissues Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
Structure Homo and heterooligomers with other chemokines. Interacts with the brown dog tick evasin-4.
Post-translational modification N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.; N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG.; The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
Target Relevance information above includes information from UniProt accession: P13501
The UniProt Consortium

Data

Immobilized Human CCL5, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).

Immobilized Human CCL5, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1µg/ml determined by ELISA.

Human CCL5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.