Human CALCA/CGRP Protein 3587

$270.00 – $900.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Ala26-Asn141

SKU: 3587parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001732
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
molecular weight	The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CALCA/CGRP Protein 3587

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
Protein names Calcitonin gene-related peptide 1 (Alpha-type CGRP) (Calcitonin gene-related peptide I) (CGRP-I)
Protein family Calcitonin family
Mass 13899Da
Function CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP. {ECO:0000269\|PubMed:1318039}.
Subellular location Secreted.
Target Relevance information above includes information from UniProt accession: P06881
The UniProt Consortium

The purity of Human CALCA/CGRP is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CALCA/CGRP Protein

Human CALCA/CGRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. Question: What are the key features of the Human CALCA (CGRP) Protein available on benchmarkantibodies.com?
Answer: The Human CALCA (CGRP) Protein on our website is a highly purified recombinant protein that is suitable for various research applications. It is expressed in E. coli and processed to ensure high bioactivity and purity for reliable experimental results.

2. Question: How should I store the Human CALCA (CGRP) Protein for optimal stability and shelf life?
Answer: To maintain the stability and bioactivity of the Human CALCA (CGRP) Protein, we recommend storing it at -80°C upon arrival. For short-term storage of up to one month, it can be kept at -20°C. Avoid repeated freeze-thaw cycles to prevent protein degradation.

3. Question: Is the Human CALCA (CGRP) Protein compatible with specific assays or experimental techniques?
Answer: Yes, the Human CALCA (CGRP) Protein is compatible with various immunoassays, cell culture studies, and biochemical assays that require a reliable source of recombinant CALCA protein. It can be used in receptor binding assays, functional studies, and other research applications related to its biological activity.

4. Question: What applications is the Human CALCA (CGRP) Protein commonly used for in research?
Answer: The Human CALCA (CGRP) Protein is widely used in studies related to neuropeptide signaling, pain research, migraine pathophysiology, and neurobiology. Researchers often use this protein in in vitro and in vivo experiments to investigate the role of CALCA in various physiological and pathological processes.

5. Question: Are there any specific handling precautions or recommendations when working with the Human CALCA (CGRP) Protein?
Answer: When handling the Human CALCA (CGRP) Protein, it is important to use appropriate personal protective equipment and follow good laboratory practices to avoid contamination. Additionally, we recommend aliquoting the protein into smaller portions to avoid repeated freeze-thaw cycles and to minimize the risk of protein degradation.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.