ELISA with Human c-MPL/Thrombopoietin R Protein 3589

Human c-MPL/Thrombopoietin R Protein 3589

$345.00 – $1,150.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln26-Trp491

SKU: 3589parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P40238
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
molecular weight	The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human c-MPL/Thrombopoietin R Protein 3589

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
Protein names Thrombopoietin receptor (TPO-R) (Myeloproliferative leukemia protein) (Proto-oncogene c-Mpl) (CD antigen CD110)
Protein family Type I cytokine receptor family, Type 1 subfamily
Mass 71245Da
Function Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses. {ECO:0000250\|UniProtKB:Q08351}.
Subellular location Cell membrane {ECO:0000269\|PubMed:25538044}; Single-pass type I membrane protein. Golgi apparatus {ECO:0000269\|PubMed:25538044}. Cell surface {ECO:0000269\|PubMed:25538044}.
Tissues Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
Structure Homodimer (PubMed:25538044). Interacts with ATXN2L. {ECO:0000269\|PubMed:11784712, ECO:0000303\|PubMed:25538044}.
Post-translational modification Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. {ECO:0000269\|PubMed:19880496}.
Domain The WSXWS motif appears to be necessary for proper protein folding and thereby efficient int
Target Relevance information above includes information from UniProt accession: P40238
The UniProt Consortium

Data

ELISA with Human c-MPL/Thrombopoietin R Protein

Immobilized Human c-MPL, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

HPLC of Human c-MPL/Thrombopoietin R Protein

The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human c-MPL/Thrombopoietin R Protein

Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.