ELISA with Human c-MPL/Thrombopoietin R Protein 3589

Human c-MPL/Thrombopoietin R Protein 3589

$345.00 – $1,150.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln26-Trp491

available size

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SKU: 3589parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P40238
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
molecular weight	The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human c-MPL/Thrombopoietin R Protein 3589

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
Protein names Thrombopoietin receptor (TPO-R) (Myeloproliferative leukemia protein) (Proto-oncogene c-Mpl) (CD antigen CD110)
Gene names MPL,MPL TPOR
Protein family Type I cytokine receptor family, Type 1 subfamily
Mass 71245Da
Function FUNCTION: Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed:15899890, PubMed:37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production. {ECO:0000269\|PubMed:15899890, ECO:0000269\|PubMed:37633268}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:15899890, ECO:0000269\|PubMed:25538044, ECO:0000269\|PubMed:32029621}; Single-pass type I membrane protein. Golgi apparatus {ECO:0000269\|PubMed:25538044}. Cell surface {ECO:0000269\|PubMed:25538044}.
Tissues TISSUE SPECIFICITY: Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
Structure SUBUNIT: Homodimer (PubMed:32029621, PubMed:25538044). Interacts with ATXN2L (PubMed:11784712). Interacts with JAK2 and TYK2; these interactions increase MPL localization to the cell membrane (PubMed:15899890). Interacts with THPO (PubMed:37633268). Interacts with SHIP/INPP5D (PubMed:24607955). Interacts with BTK (PubMed:24607955). Interacts with SYK; this interaction negatively regulates THPO-mediated ERK1/2 signaling (PubMed:24607955). {ECO:0000269\|PubMed:11784712, ECO:0000269\|PubMed:15899890, ECO:0000269\|PubMed:24607955, ECO:0000269\|PubMed:25538044, ECO:0000269\|PubMed:32029621, ECO:0000269\|PubMed:37633268}.
Post-translational modification PTM: Phosphorylated at Tyr-591 in response to THPO stimulation. {ECO:0000269\|PubMed:24607955}.; PTM: Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. {ECO:0000269\|PubMed:19880496}.
Domain DOMAIN: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.; DOMAIN: The box 1 motif is required for JAK interaction and/or activation.
Target Relevance information above includes information from UniProt accession: P40238
The UniProt Consortium

Data

ELISA with Human c-MPL/Thrombopoietin R Protein

Immobilized Human c-MPL, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

HPLC of Human c-MPL/Thrombopoietin R Protein

The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human c-MPL/Thrombopoietin R Protein

Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.