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Human BTN2A1&BTN3A1 complex Protein 5023

$375.00$1,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q7KYR7(BTN2A1)&O00481(BTN3A1)

express system

HEK293

product tag

C-His, C-Flag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.

molecular weight

The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human BTN2A1&BTN3A1 complex Protein 5023

Data

ELISA with Human BTN2A1&BTN3A1 complex Protein
Immobilized Human BTN3A1&BTN2A1, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
HPLC of Human BTN2A1&BTN3A1 complex Protein
The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human BTN2A1&BTN3A1 complex Protein
Human BTN2A1&BTN3A1 complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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