Human Beta-NGF Protein 2143

$225.00 – $750.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Ser122-Ala241

SKU: 2143parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P01138
express system	HEK293
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE
background	Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).
molecular weight	The protein has a predicted MW of 13.49 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Beta-NGF Protein 2143

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).
Protein names Beta-nerve growth factor (Beta-NGF)
Gene names NGF,NGF NGFB
Protein family NGF-beta family
Mass 9606Da
Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
Catalytic activity BINDING 173; /ligand="a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol)"; /ligand_id="ChEBI:CHEBI:64771"; /ligand_note="ligand shared between dimeric partners"; /note="in other chain"; /evidence="ECO:0000250\|UniProtKB:P01139"; BINDING 209; /ligand="a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol)"; /ligand_id="ChEBI:CHEBI:64771"; /ligand_note="ligand shared between dimeric partners"; /evidence="ECO:0000250\|UniProtKB:P01139"; BINDING 209; /ligand="a 1-acyl-sn-glycero-3-phospho-L-serine"; /ligand_id="ChEBI:CHEBI:64379"; /ligand_note="ligand shared between dimeric partners"; /evidence="ECO:0000250\|UniProtKB:P01139"
Subellular location Secreted. Endosome lumen. Note=ProNGF is endocytosed after binding to the cell surface receptor formed by SORT1 and NGFR.
Structure Homodimer (PubMed:10490030, PubMed:15131306, PubMed:17196528). The homodimer interacts with a single NTRK1 chain (PubMed:10490030, PubMed:17196528). The homodimer interacts with a single NGFR chain (PubMed:15131306). The NGF dimer interacts with a single SORCS2 chain (via extracellular domain) (By similarity). The NGF precursor (proNGF) binds to a receptor complex formed by SORT1 and NGFR, which leads to NGF endocytosis. Both mature NGF and the immature NGF precursor (proNGF) interact with SORCS2 and with the heterodimer formed by SORCS2 and NGFR (via extracellular domains) (By similarity). The NGF precursor (proNGF) has much higher affinity for SORCS2 than mature NGF. The NGF precursor (proNGF) has much higher affinity for SORT1 than mature NGF (By similarity). Interacts with ADAM10 in a divalent cation-dependent manner (PubMed:20164177).
Target Relevance information above includes information from UniProt accession: P01138
The UniProt Consortium

Cell based assay with Human Beta-NGF Protein

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2 - 2 ng/ml.

Human Beta-NGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.