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Human BAFF/TNFSF13B/CD257 Trimer Protein 4486

$330.00$1,100.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Thr141-Leu285
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9Y275

express system

HEK293

product tag

N-His-Flag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

molecular weight

The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human BAFF/TNFSF13B/CD257 Trimer Protein 4486

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Protein names
Tumor necrosis factor ligand superfamily member 13B (B lymphocyte stimulator) (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1) (CD antigen CD257) [Cleaved into: Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis
Gene names
TNFSF13B,TNFSF13B BAFF BLYS TALL1 TNFSF20 ZTNF4 UNQ401/PRO738
Protein family
Tumor necrosis factor family
Mass
31223Da
Function
FUNCTION: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. {ECO:0000269|PubMed:10973284}.; FUNCTION: Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. {ECO:0000250}.; FUNCTION: [Isoform 3]: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. {ECO:0000269|PubMed:10973284}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted.
Tissues
TISSUE SPECIFICITY: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. {ECO:0000269|PubMed:12867412}.
Structure
SUBUNIT: Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. {ECO:0000269|PubMed:12867412, ECO:0000269|PubMed:22749832}.
Post-translational modification
PTM: The soluble form derives from the membrane form by proteolytic processing.; PTM: Isoform 2 is not efficiently shed from the membrane unlike isoform 1. {ECO:0000250}.; PTM: N-glycosylated.
Domain
TOPO_DOM 1
Target Relevance information above includes information from UniProt accession : Q9Y275
The UniProt Consortium

Data

SPR with Human BAFF/TNFSF13B/CD257 Trimer Protein
Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).
ELISA with Human BAFF/TNFSF13B/CD257 Trimer Protein
Immobilized Human BAFF Trimer, His Tag at 2µg/ml (100µl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).
ELISA with Human BAFF/TNFSF13B/CD257 Trimer Protein
Immobilized Human BAFF Trimer, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
HPLC of Human BAFF/TNFSF13B/CD257 Trimer Protein
The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human BAFF/TNFSF13B/CD257 Trimer Protein
Human BAFF Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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