SPR with Human B7-H6/NCR3LG1 Protein 4254

ELISA with Human B7-H6/NCR3LG1 Protein 4254

Human B7-H6/NCR3LG1 Protein 4254

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp25-Ser262

SKU: 4254parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q68D85
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
molecular weight	The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human B7-H6/NCR3LG1 Protein 4254

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Protein names Natural cytotoxicity triggering receptor 3 ligand 1 (B7 homolog 6) (B7-H6)
Mass 50827Da
Function Triggers NCR3-dependent natural killer cell activation. {ECO:0000269\|PubMed:19528259}.
Subellular location Cell membrane {ECO:0000269\|PubMed:19528259}; Single-pass type I membrane protein {ECO:0000269\|PubMed:19528259}.
Tissues Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). {ECO:0000269\|PubMed:19528259}.
Structure Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1. {ECO:0000269\|PubMed:19528259, ECO:0000269\|PubMed:21422170}.
Domain The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be
Target Relevance information above includes information from UniProt accession: Q68D85
The UniProt Consortium

Data

Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 µM as determined in SPR assay (Biacore T200).

Immobilized Human NKp30, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).

The purity of Human B7-H6 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human B7-H6/NCR3LG1 Protein

Human B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.