Human B7-H4 Protein 4226

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe29-Ala258

SKU: 4226parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q7Z7D3
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .
molecular weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human B7-H4 Protein 4226

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease statusÂ .
Protein names V-set domain-containing T-cell activation inhibitor 1 (B7 homolog 4) (B7-H4) (B7h.5) (Immune costimulatory protein B7-H4) (Protein B7S1) (T-cell costimulatory molecule B7x)
Protein family Immunoglobulin superfamily, BTN/MOG family
Mass 30878Da
Function Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. {ECO:0000250\|UniProtKB:Q7TSP5, ECO:0000269\|PubMed:15878339, ECO:0000269\|PubMed:16606666, ECO:0000269\|PubMed:17509674, ECO:0000269\|PubMed:17875732}.
Subellular location Cell membrane {ECO:0000255}; Single-pass type I membrane protein {ECO:0000305}. Note=Expressed at the cell surface. A soluble form has also been detected. {ECO:0000255, ECO:0000269\|PubMed:12818165, ECO:0000269\|PubMed:15878339, ECO:0000269\|PubMed:16782226}.
Tissues Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis. {ECO:0000269\|PubMed:12818165, ECO:0000269\|PubMed:14568939, ECO:0000269\|PubMed:15878339, ECO:0000269\|PubMed:16606666, ECO:0000269\|PubMed:16782226, ECO:0000269\|PubMed:16798883, ECO:0000269\|PubMed:17509674}.
Post-translational modification N-glycosylated. {ECO:0000269\|PubMed:15878339}.
Target Relevance information above includes information from UniProt accession: Q7Z7D3
The UniProt Consortium

Data

Immobilized Human B7-H4, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.

The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.

Human B7-H4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.