SPR with Human Alkaline phosphatase (Intestinal type) /ALPI Protein 4324

SDS-PAGE gel of Human Alkaline phosphatase (Intestinal type) /ALPI Prot 4324

Human Alkaline phosphatase (Intestinal type) /ALPI Protein 4324

$375.00 – $1,250.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Val20-Asp503

SKU: 4324parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P09923
express system	HEK293
product tag	N-His-Flag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
molecular weight	The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Alkaline phosphatase (Intestinal type) /ALPI Protein 4324

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Protein names Intestinal-type alkaline phosphatase (IAP) (Intestinal alkaline phosphatase) (EC 3.1.3.1)
Gene names ALPI,ALPI
Protein family Alkaline phosphatase family
Mass 9606Da
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Catalytic activity BINDING 61; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 61; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 111; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 174; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 235; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 288; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 289; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 304; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 330; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 335; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 339; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 376; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 377; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250\|UniProtKB:P15693"; BINDING 451; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250\|UniProtKB:P15693"
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor .
Structure Homodimer.
Target Relevance information above includes information from UniProt accession: P09923
The UniProt Consortium

Data

SPR with Human Alkaline phosphatase (Intestinal type) /ALPI Protein

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 µM as determined in SPR assay (Biacore T200).

HPLC of Human Alkaline phosphatase (Intestinal type) /ALPI Protein

The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Alkaline phosphatase (Intestinal type) /ALPI Prot

Human Alkaline phosphatase (Intestinal type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.