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Human Alkaline Phosphatase (Germ type) /ALPG Protein 2490

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ile20-Asp503
SKU: 2490parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P10696

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

molecular weight

The protein has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Alkaline Phosphatase (Germ type) /ALPG Protein 2490

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Protein names
Alkaline phosphatase, germ cell type (EC 3.1.3.1) (ALP-1) (Alkaline phosphatase Nagao isozyme) (Alkaline phosphatase, placental-like) (Germ cell alkaline phosphatase) (GCAP) (Placental alkaline phosphatase-like) (PLAP-like)
Gene names
ALPG,ALPG ALPPL ALPPL2
Protein family
Alkaline phosphatase family
Mass
9606Da
Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Catalytic activity
BINDING 61; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 61; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 111; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 174; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 235; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 288; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 289; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 304; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:P05186"; BINDING 330; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 335; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 339; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 376; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 377; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P05187"; BINDING 451; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P05187"
Subellular location
Cell membrane; Lipid-anchor, GPI-anchor.
Tissues
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
Structure
Homodimer.
Target Relevance information above includes information from UniProt accession: P10696
The UniProt Consortium

ELISA with Human Alkaline Phosphatase (Germ type) /ALPG Protein
Immobilized Human Alkaline Phosphatase (Germ Type) , hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
HPLC of Human Alkaline Phosphatase (Germ type) /ALPG Protein
The purity of Human Alkaline Phosphatase (Germ type) is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Alkaline Phosphatase (Germ type) /ALPG Protein
Human Alkaline Phosphatase (Germ type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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