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Human A2AR Protein-Nanodisc 2011

$1,100.00$3,800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met1-Ser412
SKU: 2011parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P29274

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult.

molecular weight

The protein has a predicted MW of 45.5 kDa.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human A2AR Protein-Nanodisc 2011

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult.
Protein names
Adenosine receptor A2a
Gene names
ADORA2A,ADORA2A ADORA2
Protein family
G-protein coupled receptor 1 family
Mass
9606Da
Function
Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Catalytic activity
BINDING 169; /ligand="adenosine"; /ligand_id="ChEBI:CHEBI:16335"; /ligand_note="agonist"; /evidence="ECO:0000269|PubMed:21593763, ECO:0007744|PDB:2YDO"; BINDING 253; /ligand="adenosine"; /ligand_id="ChEBI:CHEBI:16335"; /ligand_note="agonist"; /evidence="ECO:0000269|PubMed:21593763, ECO:0007744|PDB:2YDO"; BINDING 277; /ligand="adenosine"; /ligand_id="ChEBI:CHEBI:16335"; /ligand_note="agonist"; /evidence="ECO:0000269|PubMed:21593763, ECO:0007744|PDB:2YDO"; BINDING 278; /ligand="adenosine"; /ligand_id="ChEBI:CHEBI:16335"; /ligand_note="agonist"; /evidence="ECO:0000269|PubMed:21593763, ECO:0007744|PDB:2YDO"
Subellular location
Cell membrane ; Multi-pass membrane protein. Note=Colocalizes with GAS2L2 at neuronal processes.
Structure
Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct (PubMed:16339847). May interact with DRD4 (PubMed:18832607). Interacts with NECAB2 (PubMed:17689978). Interacts (via cytoplasmic C-terminal domain) with GAS2L2; interaction enhances receptor-mediated adenylyl cyclase activity (By similarity).
Post-translational modification
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
Domain
Th
Target Relevance information above includes information from UniProt accession: P29274
The UniProt Consortium

SPR with Human A2AR Protein-Nanodisc
Human A2AR Nanodisc, His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM as determined in SPR assay (Biacore T200).
ELISA with Human A2AR Protein-Nanodisc
Immobilized Human A2AR Nanodisc, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
ELISA with Human A2AR Protein-Nanodisc
Loaded Anti-A2AR Antibody on Protein A-Biosensor, can bind Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM as determined in BLI assay (Gator).

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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