ELISA with Human 4-1BB Ligand/TNFSF9 Trimer Protein 4409

Human 4-1BB Ligand/TNFSF9 Trimer Protein 4409

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg71-Glu254

SKU: 4409parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P41273
express system	HEK293
product tag	N-monomeric hFc
purity	> 95% as determined by Tris-Bis PAGE
background	The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
molecular weight	The protein has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human 4-1BB Ligand/TNFSF9 Trimer Protein 4409

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Protein names Tumor necrosis factor ligand superfamily member 9 (4-1BB ligand) (4-1BBL)
Gene names TNFSF9,TNFSF9
Protein family Tumor necrosis factor family
Mass 9606Da
Function Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
Subellular location Membrane; Single-pass type II membrane protein.
Tissues Expressed in brain, placenta, lung, skeletal muscle and kidney.
Structure Homotrimer.
Target Relevance information above includes information from UniProt accession: P41273
The UniProt Consortium

Data

ELISA with Human 4-1BB Ligand/TNFSF9 Trimer Protein

Immobilized Human 4-1BB, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.

HPLC of Human 4-1BB Ligand/TNFSF9 Trimer Protein

The purity of Human 4-1BB Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human 4-1BB Ligand/TNFSF9 Trimer Protein

Human 4-1BB Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.