FITC-Labeled Human Siglec-2/CD22 Protein 3512

$900.00 – $2,000.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Asp20-Arg687

SKU: 3512parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P20273
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE
background	CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
molecular weight	The protein has a predicted MW of 101.9 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

FITC-Labeled Human Siglec-2/CD22 Protein 3512

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
Protein names B-cell receptor CD22 (B-lymphocyte cell adhesion molecule) (BL-CAM) (Sialic acid-binding Ig-like lectin 2) (Siglec-2) (T-cell surface antigen Leu-14) (CD antigen CD22)
Gene names CD22,CD22 SIGLEC2
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 9606Da
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Subellular location Cell membrane; Single-pass type I membrane protein.
Tissues B-lymphocytes.
Structure Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1.
Post-translational modification Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.; Phosphorylated on tyrosine residues by LYN.
Domain Co
Target Relevance information above includes information from UniProt accession: P20273
The UniProt Consortium

Data

SDS-PAGE gel of FITC-Labeled Human Siglec-2/CD22 Protein

FITC-Labeled Human Siglec-2/CD22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

SKU from product: 3512parent
ABID (antibody): 3512
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SQL FAQ: SELECT `faq_html` FROM `faq_table` WHERE `abid` = '3512' LIMIT 1;
FAQ content found:

1. What is the concentration of the FITC-labeled Human Siglec-2/CD22 protein (3512)?
- The concentration of the FITC-labeled Human Siglec-2/CD22 protein (3512) is provided as 0.1 mg/mL.

2. How should I store the FITC-labeled Human Siglec-2/CD22 protein (3512)?
- The protein should be stored at -80°C upon arrival. For short-term storage, it can be kept at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.

3. Is the FITC-labeled Human Siglec-2/CD22 protein (3512) compatible with various applications?
- Yes, this protein is suitable for applications such as flow cytometry, immunofluorescence, and immunohistochemistry. It can be used for various research purposes related to Siglec-2/CD22 protein studies.

4. Does the FITC-labeled Human Siglec-2/CD22 protein (3512) come with any specific recommendations for experimental use?
- It is recommended to aliquot the protein upon receipt to avoid multiple freeze-thaw cycles. Additionally, users should refer to the provided datasheet for specific protocols and guidelines for their intended experiments.

5. Can the FITC-labeled Human Siglec-2/CD22 protein (3512) be used for both qualitative and quantitative analysis?
- Yes, this protein is suitable for qualitative analysis through techniques like immunofluorescence, as well as quantitative analysis, such as flow cytometry, based on the FITC labeling for detection and analysis.

Frequently Asked Questions

SQL Count: SELECT COUNT(*) as total FROM `references` WHERE `abid` = '3512';
Total count of publications: 0
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

No publications found.

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