FITC-Labeled Human EGFRVIII Protein 3553

$900.00 – $2,000.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Leu25-Ser378

SKU: 3553parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001333870
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE
background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
molecular weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

FITC-Labeled Human EGFRVIII Protein 3553

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

FACS with FITC-Labeled Human EGFRVIII Protein

FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5µg/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

SDS-PAGE gel of FITC-Labeled Human EGFRVIII Protein

FITC-Labeled Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Frequently Asked Questions

1. Question: What is the concentration of the FITC-labeled human EGFRvIII protein?
Answer: The concentration of the FITC-labeled human EGFRvIII protein is 0.1 mg/ml.

2. Question: How should I store the FITC-labeled human EGFRvIII protein for optimal stability?
Answer: To maintain optimal stability, store the FITC-labeled human EGFRvIII protein at -20°C upon arrival. Aliquot and store it at -80°C for long-term storage.

3. Question: Is the FITC-labeled human EGFRvIII protein compatible with common immunofluorescence techniques?
Answer: Yes, the FITC-labeled human EGFRvIII protein is compatible with common immunofluorescence techniques, making it suitable for various cellular imaging applications.

4. Question: What applications can the FITC-labeled human EGFRvIII protein be used for?
Answer: The FITC-labeled human EGFRvIII protein can be used for applications such as immunofluorescence microscopy, flow cytometry, and other fluorescence-based assays to study EGFRvIII protein localization and dynamics in cells.

5. Question: Can the FITC-labeled human EGFRvIII protein be used for live cell imaging?
Answer: Yes, the FITC-labeled human EGFRvIII protein is suitable for live cell imaging studies, enabling real-time visualization of EGFRvIII protein behavior and interactions within living cells.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.