ELISA with Cynomolgus/Rhesus macaque TIGIT Protein 4156

Cynomolgus/Rhesus macaque TIGIT Protein 4156

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met22-Ile141

SKU: 4156parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_015300911
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
molecular weight	The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus/Rhesus macaque TIGIT Protein 4156

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Protein names T-cell immunoreceptor with Ig and ITIM domains (V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)
Gene names TIGIT,TIGIT VSIG9 VSTM3
Mass 9606Da
Function Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level).
Structure Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3.
Domain Co
Target Relevance information above includes information from UniProt accession: Q495A1
The UniProt Consortium

Data

ELISA with Cynomolgus/Rhesus macaque TIGIT Protein

Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.

HPLC of Cynomolgus/Rhesus macaque TIGIT Protein

The purity of Cynomolgus/Rhesus macaque TIGIT is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus/Rhesus macaque TIGIT Protein

Cynomolgus/Rhesus macaque TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.