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Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein 4538

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Phe19-Arg238
SKU: 4538parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

G7PSE7

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

molecular weight

The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein 4538

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Protein names
CD274 molecule
Gene names
CD274,CD274
Mass
9541Da
Subellular location
SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004479}; Single-pass type I membrane protein {ECO:0000256|ARBA:ARBA00004479}.
Target Relevance information above includes information from UniProt accession : G7PSE7
The UniProt Consortium

Data

SPR with Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 1.28 µM as determined in SPR assay (Biacore T200).
ELISA with Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Immobilized Cynomolgus/Rhesus macaque PD-L1, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
HPLC of Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
The purity of Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Cynomolgus/Rhesus macaque PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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