SPR with Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein 4255

ELISA with Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein 4255

Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein 4255

$195.00 – $650.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln24-Lys265

SKU: 4255parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001035118
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
molecular weight	The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein 4255

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein

Cynomolgus/Rhesus macaque EpCAM, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-EpCAM Antibody, hFc Tag with an affinity constant of 21.40 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein

Immobilized Cynomolgus/Rhesus macaque EpCAM, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.38µg/ml determined by ELISA (QC Test).

HPLC of Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein

The purity of Cynomolgus/Rhesus macaque EpCAM is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus/Rhesus macaque EpCAM/TROP1 Protein

Cynomolgus/Rhesus macaque EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.