ELISA with Cynomolgus PDGF R beta/CD140b Protein 3119

Cynomolgus PDGF R beta/CD140b Protein 3119

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu33-Lys531

SKU: 3119parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A2K5TUS0
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Platelet-derived growth factor receptor (PDGFR) signaling is involved in proliferation and survival in a wide array of cell types.PDGFR-β signalling, via TGF-β signalling, may be crucial for restoration of BBB integrity after cerebral ischemia and therefore represents a novel potential therapeutic target.
molecular weight	The protein has a predicted MW of 57.49 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus PDGF R beta/CD140b Protein 3119

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Platelet-derived growth factor receptor (PDGFR) signaling is involved in proliferation and survival in a wide array of cell types.PDGFR-β signalling, via TGF-β signalling, may be crucial for restoration of BBB integrity after cerebral ischemia and therefore represents a novel potential therapeutic target.
Protein names Platelet-derived growth factor receptor beta (PDGF-R-beta) (PDGFR-beta) (EC 2.7.10.1) (Beta platelet-derived growth factor receptor) (Beta-type platelet-derived growth factor receptor)
Gene names PDGFRB,PDGFRB
Protein family Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamil
Mass 9541Da
Function Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells.
Catalytic activity BINDING 579; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"; BINDING 606..614; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR500948-2"; BINDING 607..614; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 634; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2, ECO:0000256\|PROSITE-ProRule:PRU10141"; BINDING 682..688; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 830; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 831; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"; BINDING 844; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"
Subellular location Cell membrane ; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. Membrane ; Single-pass type I membrane protein .
Structure Interacts with homodimeric PDGFB and PDGFD, and with heterodimers formed by PDGFA and PDGFB.
Target Relevance information above includes information from UniProt accession: A0A2K5TUS0
The UniProt Consortium

Data

ELISA with Cynomolgus PDGF R beta/CD140b Protein

Immobilized Cynomolgus PDGFR beta, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-PDGFRB Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.

HPLC of Cynomolgus PDGF R beta/CD140b Protein

The purity of Cynomolgus PDGF R beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus PDGF R beta/CD140b Protein

Cynomolgus PDGF R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.